ARUP's Laboratory Test Directory

Autoimmune Hepatitis Panel Plus with Reflex to Titers : 0055351

Mnemonic: AIH PLUS

Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
Performed: Sun-Sat
Reported: 2-5 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature: Refrigerated.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
 
Test Number Components Reference Interval
0050080Anti-Nuclear Antibodies (ANA), IgG by ELISA with Reflex to ANA, IgG by IFA None Detected
0055248F-Actin (Smooth Muscle) Antibody, IgG Effective May 16, 2011
 
19 Units or less Negative
20-30 Units Weak Positive - Suggest repeat testing in two to three weeks with fresh specimen.
31 Units or greater Positive - Suggestive of autoimmune hepatitis type 1 or chronic active hepatitis.
0055241Liver-Kidney Microsome - 1 Antibody, IgG 0.0-20.0 U: Negative
20.1-24.9 U: Equivocal
Greater than or equal to 25.0 U: Positive
0050065Mitochondrial M2 Antibody, IgG (ELISA) 20.0 Units or less: Negative
20.1-24.9 Units: Equivocal
25.0 Units or greater: Positive
0050811Anti-Neutrophil Cytoplasmic Antibody, IgG < 1:20: Not significant
0055235Soluble Liver Antigen Antibody, IgG 0.0-20.0 U: Negative
20.1-24.9 U: Equivocal
Greater than or equal to 25.0 U: Positive
 
Note: Specimens are screened for ANA using ELISA. If antibodies are detected, then an IFA titer using HEp-2 cells will be added.  Specimens are screened for ANCA by IFA on ethanol-fixed slides which allow differentiation of C and P patterns. If screen result is 1:20, then specimens will be serially diluted and tested on ethanol-fixed slides to establish titer and confirm pattern. All P-ANCA patterns are also tested on formalin-fixed slides to confirm P-ANCA by removing ANA receptor sites and maintaining cytoplasmic localization of myeloperoxidase. 
CPT Code(s): 86038 ANA; if reflexed, add 86039 ANA titer; 83516 F-Actin IgG; 86376 LKM-1 IgG; 83516 AMA; 83516 SLA IgG; 86255 ANCA IgG; if reflexed add 86256 if ANCA IgG titer
 
 

 

 

 
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