ARUP's Laboratory Test Directory

Hepatitis C Virus RNA Quantitative bDNA : 0051811

Mnemonic: HCVRNA BC

Methodology: Branched Chain DNA
Performed: Tue, Fri
Reported: 2-5 days
Specimen Required: Collect: One 7 mL serum separator tube, PPT, lavender (EDTA), or pink (K2EDTA).

Storage/Transport Temperature: 1 mL serum or plasma, frozen. (Min: 0.2 mL) Submit specimen in an ARUP Standard Transport Tube. Specimens collected in a PPT may be submitted in original container.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Separate serum or plasma from cells within four hours. Do not store PPT whole blood at 2-8°C.

Unacceptable Conditions: Nonfrozen, heparinized, thawed or refrozen specimens.

Stability (collection to initiation of testing): Ambient: 4 hours (on cells); Serum or plasma removed from cells; Refrigerated: 48 hours; Frozen
(-20°C): 3 days; (-70°C): 4 months

Reference Interval:
Not detected
Interpretive Data: The quantitative range of this assay is 2.8-6.9 log IU/mL (615-7,690,000 IU/mL).

An interpretation of "Not Detected" does not rule out the presence of inhibitors in the patient specimen or hepatitis C virus RNA concentrations below the level of detection of the assay.  Care should be taken in the interpretation of any single viral load determination.  Low positive values may occasionally be seen in specimens from patients who are uninfected. 

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).

Note: This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis.  This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in HCV RNA levels.  A qualitative PCR assay with a detection limit of 1.7 log IU/mL (50 IU/mL), Hepatitis C Virus RNA Qualitative PCR (0098264), is available for the assessment of lower levels of HCV viremia.
CPT Code(s): 87522
 
 

 

 

 
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