ARUP's Laboratory Test Directory

Microsatellite Instability (MSI), HNPCC/Lynch Syndrome, by PCR : 0051740
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Additional Technical Information
  


Mnemonic: MSI PCR

Ordering Recommendation: Order on tumor and normal tissue to distinguish microsatellite unstable (MSI) from microsatellite stable tumors.
Methodology: Polymerase Chain Reaction/Fragment Analysis
Performed: Varies
Reported: 10-14 days
Specimen Required: Collect: Tumor AND normal epithelial tissue.

Specimen Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block(s) or 10 unstained 5-micron slides (5 tumor and 5 normal epithelial). (Min: 3 tumor tissue and 3 normal epithelial tissue slides) Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature: Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Remarks: Include surgical pathology report. Tissue block will be returned after testing.

Unacceptable Conditions: Specimens fixed/processed in alternative fixatives (alcohol, Prefer®). Frozen specimens. Decalcified specimens. Less than 25 percent tumor or less than 50 percent normal epithelial tissue.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 88381, 81301, G0452
Cross References: Lynch Syndrome/Microsatellite Instability (HNPCC/Lynch Syndrome, Microsatellite Instability by PCR), Microsatellite Instability/HNPCC (HNPCC/Lynch Syndrome, Microsatellite Instability by PCR), MSI, Microsatellite Instability (HNPCC/Lynch Syndrome, Microsatellite Instability by PCR)