| Methodology: |
Cell Culture/Enzyme-Linked Immunosorbent Assay
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| Performed: |
Tue, Thu, Sat
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| Reported: |
2-5 days
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| Specimen Required: |
Collect: 1 mL whole blood in each of three Cellestis QuantiFERON®-TB Gold In Tube collection tubes (Nil, TB, and Mitogen tubes - ARUP Supply #45112). High Altitude collection tubes (ARUP Supply #46261).
Storage/Transport Temperature: Original collection tubes, after incubation and centrifugation, at 2-8°C.
Remarks: Immediately following collection, each specimen tube must be mixed vigorously by shaking the tube up and down 10 times to ensure that the entire inner surface of the tube has been coated with blood.
After mixing, incubate upright at 37°C for 16-24 hours (within 16 hours of collection).
After incubation, centrifuge for 15 minutes at 2000-3000 RCF (g).
Unacceptable Conditions: Specimens that have not been centrifuged. Ambient specimens.
Stability (collection to initiation of testing): After incubation and centrifugation: Ambient: 2 hours; Refrigerated: 4 weeks; Frozen: 4 months
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