ARUP's Laboratory Test Directory

Kell K/k Antigen (KEL) Genotyping : 0051644
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Time Sensitive
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Consent Form Recommended


Mnemonic: KEL

Methodology: Polymerase Chain Reaction/Fluorescence Monitoring
Performed: Mon, Thu
Reported: 2-7 days
Specimen Required: Collect: Fetal Specimen: Two T-25 flasks at 80% confluency of cultured amniocytes. If the client is unable to culture amniocytes, this can be arranged by contacting ARUP Client Services at (800) 522-2787. Or amniotic fluid.
AND Maternal Cell Contamination Specimen: Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

OR Whole Blood in lavender (EDTA), pink(K2EDTA), or yellow (ACD Solution A or B).

Specimen Preparation: Cultured Amniocytes: Fill flasks with culture media. Transport two T-25 flasks at 80% confluency of cultured amniocytes filled with culture media. Backup cultures must be retained the client's institution until testing is complete.
OR Amniotic Fluid: Transport 1 mL unspun fluid. (Min: 1 mL)
AND Maternal Cell Contamination Specimen: Transport 3 mL whole blood (Min: 1 mL)

OR Whole Blood: Transport 3 mL whole blood (Min: 1 mL)

Storage/Transport Temperature: Cultured Amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to liability of cells.
Amniotic fluid: Room temperature.
Whole Blood or Maternal Cell Contamination Specimen: Room temperature.

Remarks: Maternal specimen is recommended for proper test interpretation. Order Maternal Cell Contamination. Patient History Form is available on the ARUP Web site or by contacting ARUP Client Services.

Stability (collection to initiation of testing): Fetal Specimen: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Whole Blood or Maternal Cell contamination Specimen: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Interpretive Data: Background information: Kell K/k Antigen (KEL) Genotyping:
Characteristics: Fetal or neonatal hemolytic anemia, erythroblastosis and hydrops.
K Allele Frequency: 9 percent of Caucasians, 2 percent of African Americans; unknown for other ethnic groups.
Inheritance: Autosomal dominant.
Cause: Maternal-fetal or transfusion-related K antigen incompatibility.
Polymorphism detected: K (c.578C>T; p.Thr193Met) and k (common allele) in the KEL gene.
Clinical Sensitivity: 99 percent.
Methods: PCR of KEL gene followed by fluorescent monitoring.
Analytic Sensitivity and Specificity: 99 percent.
Limitations: Bloody amniotic fluid samples may give false-negative results because of maternal cell contamination. Specificity may be compromised by primer site mutations.

For quality assurance purposes, ARUP Laboratories will confirm the above result at no charge following delivery. Order Confirmation of Fetal Testing and include a copy of the original fetal report (or the mother's name and date of birth) with the test submission. Please contact an ARUP genetic counselor at (800) 242-2787 extension 2141 prior to specimen submission.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc



Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.

Refer to Statement C under Testing Information at http://www.aruplab.com.
Note: Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com
CPT Code(s): 83891 Isolation; 83898 Amplification; 83896 x2 Nucleic acid probes; 83912 Interpretation and report. Additional CPT code modifiers may be required for procedures preformed to test for oncologic or inherited disorders.
 
 

 

 

 
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