ARUP's Laboratory Test Directory

Toll-Like Receptor Function Assay : 0051589

Mnemonic: TLR

Methodology: Cell Culture/Quantitative Multi-Analyte Fluorescence Detection
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation: Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE CELLS REQUIRED.
Infant Minimum: 3 mL whole blood (patient) AND 7 mL whole blood (control).


Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: PAM3CSK4, synthetic bacterial lipoprotein (TLR2-TLR1 ligand), Zymosan Cell wall particles from Saccharomyces cerevisiae (TLR6-TLR2 ligand), Poly (I:C), synthetic analog of dsRNA (TLR3 ligand), Lipopolysaccharide Ultra pure S. minnesota LPS (TLR4 ligand), Flagellin, purified from S. thyphimurium (TLR5 ligand), Loxoribine, guanosine analog (TLR7 ligand) are tested independently in peripheral blood mononuclear cell (PBMC) culture. PBMC cytokine production responses to these mitogens are determined by multi-analyte fluorescence detection.

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note: Results for TNF alpha, IL-1 beta, and IL-6 are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x6 Lymphocyte transformation; 83520 x3 Cytokines
 
 

 

 

 
All ARUP Sites:        www.aruplab.com     ·     www.arupconsult.com     ·     www.arup.utah.edu     ·     www.childx.org     ·     www.utahblood.org