ARUP's Laboratory Test Directory

Toll-Like Receptor Function Assay : 0051589
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Additional Technical Information
  


Mnemonic: TLR

Methodology: Cell Culture/Quantitative Multiplex Bead Assay
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect: Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation: Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE CELLS REQUIRED.
Infant Minimum: 3 mL whole blood (patient) AND 7 mL whole blood (control).


Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:
By report
Interpretive Data: Toll-like receptors (TLR), PAM3CSK4, synthetic bacterial lipoprotein (TLR2-TLR1 ligand); zymosan cell wall particles from Saccharomyces cerevisiae (TLR6-TLR2 ligand); poly(I:C), synthetic analog of dsRNA (TLR3 ligand); lipopolysaccharide ultra pure S. minnesota LPS (TLR4 ligand); flagellin purified from S. thyphimurium (TLR5 ligand); and CL097 imidazoquinoline compound (TLR7-TLR8 ligand), are tested independently in peripheral blood mononuclear cell (PBMC) culture. PBMC cytokine production responses to these mitogens are determined by multi-analyte fluorescence detection.

See Compliance Statement B: www.aruplab.com/CS
Note: Results for TNF alpha, IL-1 beta, and IL-6 are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x6; 83520 x3