ARUP's Laboratory Test Directory

Toll-Like Receptor Function Assay : 0051589

Mnemonic: TLR

Methodology: Cell Culture/ Multi-Analyte Fluorescence Detection
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen.

Collect: One 10 mL yellow (ACD Solution A) and one normal control in a 10 mL yellow (ACD Solution A) from a healthy unrelated individual.

Storage/Transport Temperature: 10 mL whole blood and 10 mL normal control in original collection tubes at 20-25°C. (Min: 7 mL whole blood and 7 mL normal control; Infant min: 3 mL whole blood and 7 mL normal control). LIVE CELLS REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze.

Remarks: Patient and control samples must be collected within 48 hours of test performance.

Unacceptable Conditions: Refrigerated or frozen samples and samples in transport longer than 48 hours

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: PAM3CSK4, synthetic bacterial lipoprotein (TLR2-TLR1 ligand), Zymosan Cell wall particles from Saccharomyces cerevisiae (TLR6-TLR2 ligand), Poly (I:C), synthetic analog of dsRNA (TLR3 ligand), Lipopolysaccharide Ultra pure S. minnesota LPS (TLR4 ligand), Flagellin, purified from S. thyphimurium (TLR5 ligand), Loxoribine, guanosine analog (TLR7 ligand) are tested independently in peripheral blood mononuclear cell (PBMC) culture. PBMC cytokine production responses to these mitogens are determined by multi-analyte fluorescence detection.

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: Results for TNF alpha, IL-1 beta, and IL-6 are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x6 Lymphocyte transformation; 83520 x3 Cytokines
 
 

 

 

 
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