ARUP's Laboratory Test Directory

0051587: Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, Monokines

[ image for: Time Sensitive]
Time Sensitive
  

Test Mnemonic: LAM MONO
Methodology: Cell Culture/Multi-Analyte Fluorescence Detection

Performed: Tue-Fri

Reported: 9-10 days

Specimen Required:  
Collect: Two 10 mL yellow (ACD Solution A) and two normal controls in 10 mL yellow (ACD Solution A) tubes from a healthy unrelated individual.

Transport: 20 mL whole blood and 20 mL normal control in sterile containers at 20-25°C.  LIVE CELLS REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze.

Pediatric Collection/Transport: 3 mL whole blood (green, sodium heparin) and 3 mL whole blood (green, sodium heparin) normal control from a healthy unrelated individual in sterile containers at 20-25°C.

Remarks: Patient and control samples must be collected within 48 hours of test performance.

Unacceptable Conditions: Refrigerated or frozen specimens and specimens in transport longer than 48 hours. Specimens submitted in yellow (ACD Solution B).

Stability: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:
 
Available Separately Associated Tests Reference Interval
0096056Lymphocyte Antigen & Mitogen Proliferation Panel By report
0051580Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 1 beta By report
0051581Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 6 By report
0051582Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 By report
0051583Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Tumor Necrosis Factor alpha By report

Interpretive Data:
Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection.

Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.



Note:
Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.

CPT Code(s):
86353 x 5 Lymphocyte transformation; 83520 x 4 Cytokines