ARUP's Laboratory Test Directory
| 0051585: Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, TH1 Cytokines |
![[ image for: Time Sensitive]](icon_1.jpg){kind=icon} | Time Sensitive |
| Test Mnemonic: LAM TH1 CY | |
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Methodology: Cell Culture/Multi-Analyte Fluorescence Detection
Performed: Tue-Fri Reported: 9-10 days |
| Specimen Required: | |
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Collect: Two 10 mL yellow (ACD Solution A) and two normal controls in 10 mL yellow (ACD Solution A) tubes from a healthy unrelated individual.
Transport: 20 mL whole blood and 20 mL normal control in sterile containers at 20-25°C. LIVE CELLS REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze. Pediatric Collection/Transport: 3 mL whole blood (green, sodium heparin) and 3 mL whole blood (green, sodium heparin) normal control from a healthy unrelated individual in sterile containers at 20-25°C. Remarks: Patient and control samples must be collected within 48 hours of test performance. Unacceptable Conditions: Refrigerated or frozen specimens and specimens in transport longer than 48 hours. Specimens submitted in yellow (ACD Solution B). Stability: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable |
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| Interpretive Data: | |
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Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection. Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL. Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders. Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
| Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director. |
| CPT Code(s): | |
| 86353 x 5 Lymphocyte transformation; 83520 x 4 Cytokines |