ARUP's Laboratory Test Directory

Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, 12 Cytokines : 0051584
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: LAM CYT 12

Methodology: Cell Culture/Multi-Analyte Fluorescence Detection
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen.

Collect: Two 10 mL yellow (ACD Solution A) and two normal controls in 10 mL yellow (ACD Solution A) tubes from a healthy unrelated individual.

Storage/Transport Temperature: 20 mL whole blood and 20 mL normal control in original collection tubes at 20-25°C. (Min: 14 mL whole blood and 14 mL normal control; Infant min: 3 mL whole blood and 14 mL normal control). LIVE CELLS REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze.

Remarks: Patient and control samples must be collected within 48 hours of test performance.

Unacceptable Conditions: Refrigerated or frozen specimens and specimens in transport longer than 48 hours. Specimens submitted in yellow (ACD Solution B).

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
Test Number Components Reference Interval
0096056Lymphocyte Antigen & Mitogen Proliferation Panel By report
0051574Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interferon gamma By report
0051580Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 1 beta By report
0051571Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 By report
0051572Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 Receptor (Soluble) By report
0051576Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 4 By report
0051577Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 5 By report
0051581Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 6 By report
0051582Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 By report
0051578Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 10 By report
0051573Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 12 By report
0051579Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 13 By report
0051583Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Tumor Necrosis Factor alpha By report

Interpretive Data: Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection.

Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x 5 Lymphocyte transformation; 83520 x 12 Cytokines
 
 

 

 

 
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