ARUP's Laboratory Test Directory

Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 : 0051582
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Time Sensitive
  


Mnemonic: IL8 L DO

Methodology: Cell Culture/Quantitative Multiplex Bead Assay
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen.

Collect: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control samples must be collected within 48 hours of test performance.

Specimen Preparation: Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL whole blood (patient) AND 7 mL whole blood (control)). LIVE CELLS REQUIRED. Do not refrigerate or freeze.
Infant Minimum: 3 mL whole blood (patient) AND 7 mL whole blood (control).

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Frozen or refrigerated specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: Phytohemagglutinin, concanavalin A, and pokeweed mitogen are tested independently in lymphocyte culture.  Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by multi-analyte fluorescence detection.  Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.
Note: Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x3; 83520
Cross References: IL-8 (Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8)