ARUP's Laboratory Test Directory

Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 6 : 0051581
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: IL6 L DO

Methodology: Cell Culture/Multi-Analyte Fluorescence Detection
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Collect:  One 10 mL yellow (ACD Solution A) and one normal control in a 10 mL yellow (ACD Solution A) from a healthy unrelated individual.

Transport:  10 mL whole blood and 10 mL normal control in sterile containers at 20-25°C.  LIVE CELLS REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze.

Pediatric Collection/Transport:  3 mL whole blood (green, sodium heparin) and 3 mL whole blood (green, sodium heparin) normal control from a healthy unrelated individual in sterile containers at 20-25°C.

Remarks:  Patient and control samples must be collected within 48 hours of test performance.

Unacceptable Conditions:  Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability:  Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: Phytohemagglutinin, concanavalin A, and pokeweed mitogen are tested independently in lymphocyte culture.  Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by multi-analyte fluorescence detection.  Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x3 Lymphocyte transformation; 83520 Cytokine
86353 x3 Lymphocyte transformation; 83520 Cytokine
 
 

 

 

 
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