ARUP's Laboratory Test Directory

Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, TH1 Cytokines : 0051558
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: CYT TH1 LY

Methodology: Cell Culture/Quantitative Multiplex Bead Assay
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen.

Collect: Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control samples must be collected within 48 hours of test performance.

Specimen Preparation: Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL whole blood (patient) AND 7 mL whole blood (control)). LIVE CELLS REQUIRED. Do not refrigerate or freeze.
Infant Minimum: 3 mL whole blood (patient) AND 7 mL whole blood (control).

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Yellow (ACD Solution B). Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
Effective May 20, 2013
Test Number Components Reference Interval
0051571Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 By report
0051572Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 Receptor (Soluble) By report
Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 12 By report
0051574Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interferon gamma By report

Interpretive Data: Phytohemagglutinin, concanavalin A, and pokeweed mitogen are tested independently in lymphocyte culture. Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by multi-analyte fluorescence detection. Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

See Compliance Statement B: www.aruplab.com/CS
Note: Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x3; 83520 x4
Cross References: IFN gamma (Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, TH1 Cytokine, IL-12 (Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, TH1 Cytokines), IL-2 (Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, TH1 Cytokines), IL-2R (Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, TH1 Cytokines)