ARUP's Laboratory Test Directory

Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, 12 Cytokines : 0051540
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: CYT 12 LY

Methodology: Cell Culture/Multi-Analyte Fluorescence Detection
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen.

Collect: One 10 mL yellow (ACD Solution A) and one normal control in a 10 mL yellow (ACD Solution A) from a healthy unrelated individual.

Storage/Transport Temperature: 10 mL whole blood and 10 mL normal control in original collection tubes at 20-25°C. (Min: 7 mL whole blood and 7 mL normal control; Infant min: 3 mL whole blood and 7 mL normal control). LIVE CELLS REQUIRED. CRITICAL AMBIENT.
Do not refrigerate or freeze.

Remarks: Patient and control samples must be collected within 48 hours of test performance.

Unacceptable Conditions: Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
Test Number Components Reference Interval
0051571Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 By report
0051572Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 Receptor (Soluble) By report
0051573Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 12 By report
0051574Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interferon gamma By report
0051576Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 4 By report
0051577Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 5 By report
0051578Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 10 By report
0051579Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 13 By report
0051580Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 1 beta By report
0051581Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 6 By report
0051582Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 By report
0051583Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Tumor Necrosis Factor alpha By report

Interpretive Data: Phytohemagglutinin, concanavalin A, and pokeweed mitogen are tested independently in lymphocyte culture. Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by multi-analyte fluorescence detection. Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s): 86353 x3 Lymphocyte transformation; 83520 x12 Cytokines
 
 

 

 

 
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