ARUP's Laboratory Test Directory
| 0051540: Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, 12 Cytokines |
![[ image for: Time Sensitive]](icon_1.jpg){kind=icon} | Time Sensitive |
| Test Mnemonic: CYT 12 LY | |
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Methodology: Cell Culture/Multi-Analyte Fluorescence Detection
Performed: Tue-Fri Reported: 9-10 days |
| Specimen Required: | |
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Collect: One 10 mL yellow (ACD Solution A) and one normal control in a 10 mL yellow (ACD Solution A) from a healthy unrelated individual.
Transport: 10 mL whole blood and 10 mL normal control in sterile containers at 20-25°C. LIVE CELLS REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze. Pediatric Collection/Transport: 3 mL whole blood (green, sodium heparin) and 3 mL whole blood (green, sodium heparin) normal control from a healthy unrelated individual in sterile containers at 20-25°C. Remarks: Patient and control samples must be collected within 48 hours of test performance. Unacceptable Conditions: Refrigerated or frozen specimens. Specimens in transport longer than 48 hours. Stability: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable |
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| Interpretive Data: | |
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Phytohemagglutinin, concanavalin A, and pokeweed mitogen are tested independently in lymphocyte culture. Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by multi-analyte fluorescence detection. Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.
Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
| Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director. |
| CPT Code(s): | |
| 86353 x3 Lymphocyte transformation; 83520 x12 Cytokines |