ARUP's Laboratory Test Directory

Platelet Antigen 1 Genotyping (HPA-1) : 0051309

Mnemonic: HPA1

Methodology: Polymerase Chain Reaction/Fluorescence Monitoring
Performed: Mon, Thu
Reported: 2-7 days
Specimen Required: Collect: Two T-25 flasks at 80% confluency of cultured amniocytes. If the client is unable to culture amniocytes, this can be arranged by contacting ARUP Client Services at (800) 522-2787. Or amniotic fluid.
OR Whole Blood in lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

Specimen Preparation: Cultured Amniocytes: Fill flasks with culture media. Transport two T-25 flasks at 80% confluency of cultured amniocytes. Backup cultures must be retained at the client's institution until testing is complete.
OR Amniotic Fluid: Transport 10 mL unspun fluid. (Min: 5 mL)
OR Whole Blood: Transport 3 mL whole blood.

Storage/Transport Temperature: Cultured Amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to lability of cells.
Amniotic Fluid: Room temperature.
Whole Blood: Room temperature.

Stability (collection to initiation of testing): Fetal Specimen: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: Background Information for Platelet Antigen 1 Genotyping (HPA-1):
Characteristics: Spontaneous fetal intracranial bleeding in 20 percent of severe PAT affected pregnancies; risk of fetal death. Post-transfusion purpura in transfusion recipients with antibodies to a specific platelet antigen.
Incidence: Perinatal alloimmune thrombocytopenia (PAT) occurs in 1 in 5000 births.
Inheritance: For women homozygous for a rare b HPA allele with antibodies to the common a allele, there is a 50 percent risk a pregnancy will be affected if her partner is heterozygous for a allele and 100 percent risk if her partner is homozygous for a allele.
Mutations Tested: HPA-1 a (common) and b (variant) alleles.
Limitations: Mutations other than the one examined will not be detected; furthermore, mutations within the primer or probe regions could affect this assay.
Sensitivity: 99 percent
Informed consent: Recommended; forms are available at www.aruplab.com.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.



Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.

Refer to Statement C under Testing Information at http://www.aruplab.com.
CPT Code(s): 83891 Isolation; 83898 Amplification; 83896 x2 Nucleic acid probes; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.
 
 

 

 

 
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