ARUP's Laboratory Test Directory

Heparin-Induced Thrombocytopenia Antibodies with Reflex to Serotonin Release Assay : 0051249

Mnemonic: PLT HAT R

Methodology: Enzyme-Linked Immunosorbent Assay/Serotonin Release Assay
Performed: Sun-Sat
Reported: 1-10 days
Specimen Required: Collect:  One 7 mL plain red.

Transport:  3 mL serum, frozen. (Min: 2 mL) Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport:  2 mL serum, frozen. Submit specimen in an ARUP Standard Transport Tube.

Remarks:  CRITICAL FROZEN. Ship on dry ice.

Unacceptable Conditions:  Plasma or contaminated specimens. Hemolyzed, icteric, lipemic, or heat-inactivated sera may give inconsistent results.

Stability:  After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month
Reference Interval:
 
Test Number Components Reference Interval
0051052Heparin-Induced Thrombocytopenia Antibodies 0.399 OD or less
0093196Serotonin Release Assay  Heparin Dependent Platelet Antibody By report.

Interpretive Data: By itself, a positive result on this ELISA test is relatively non-specific for a clinical diagnosis of heparin-associated antibody syndrome (HIT) (frequent false-positives) and a negative result does not exclude a diagnosis of HIT if the clinical suspicion remains high (occasional false-negatives). Results should be used in conjunction with clinical findings, platelet counts and other laboratory results.

For a clinical scoring system to assess pretest probability of HIT and other guidance for diagnosing HIT, refer to the Heparin-Associated Antibody Syndrome topic at www.arupconsult.com.
CPT Code(s): 86022; if reflexed add 86022 x2
 
 

 

 

 
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