ARUP's Laboratory Test Directory
| 0051001: Glomerular Basement Membrane Antibody Panel |
| Test Mnemonic: GBM PAN | |
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Methodology: Multi-Analyte Fluorescent Detection/Indirect Fluorescent Antibody
Performed: Mon-Fri Reported: 1-5 days |
| Specimen Required: | |
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Collect: One 4 mL SST.
Transport: Two 1 mL aliquots of serum at 2-8°C. (Min: 0.5 mL per aliquot) Submit specimen in two ARUP Standard Transport Tubes. Pediatric Collection/Transport: 1 mL aliquot of serum at 2-8°C. Remarks: Separate serum from cells ASAP. Stability: Refer to individual components. |
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| Interpretive Data: | |
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The anti-GBM IFA methodology has been shown to be less specific than the quantitative tests for circulating antibodies such as the Glomerular Basement Membrane Antibody, IgG by Multi-Analyte Fluorescent Detection (0051000). The antibody used in this assay, although directed to human IgG F(ab')2, can bind to light chain sites common to all immunoglobulins causing it to be more sensitive but less specific. The determination of a positive result is based upon a diffuse "linear" staining pattern as viewed through a fluorescent microscope, all positive results should be confirmed by quantitative tests and/or renal biopsy.
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| CPT Code(s): | |
| 83516 GBM ELISA; 86255 GBM IgA; 86255 GBM IgG |