ARUP's Laboratory Test Directory

Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay : 0051000

Mnemonic: GBM-G ELIS

Methodology: Semi-Quantitative Multiplex Bead Assay
Performed: Sun-Sat
Reported: 1-2 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature: Refrigerated.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
Negative: 19 AU/mL or less
Equivocal: 20-25 AU/mL
Positive: 26 AU/mL or greater
Interpretive Data: The presence of anti-glomerular basement membrane (GBM) antibodies by Multiplex Bead Assay, may aid in the diagnosis of Goodpasture syndrome.  False positive results may occur due to reactivity against other chains of type IV collagen.  If Multiplex Bead Assay is negative but there is strong suspicion for disease, renal biopsy may be indicated.  A renal biopsy may also be essential in suspected Goodpasture disease with renal involvement, allowing diagnostic confirmation and assessment of renal prognosis.
CPT Code(s): 83516
Cross References: Anti-GBM Antibody (Glomerular Basement Membrane Antibody, IgG by Multi-Analyte Fluorescent Detection) , Anti-Glomerular Basement Membrane Antibody (Glomerular Basement Membrane Antibody, IgG by Multi-Analyte Fluorescent Detection) , Goodpasture's Syndrome (Glomerular Basement Membrane Antibody, IgG by Multi-Analyte Fluorescent Detection)