ARUP's Laboratory Test Directory

Neuronal Nuclear Antibody (ANNA) Titer IgG by IFA, Serum : 0050912

Mnemonic: ANNAGIFA

Methodology: Semi-Quantitative Indirect Fluorescent Antibody
Performed: Wed
Reported: 1-9 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature: Frozen.

Unacceptable Conditions: Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval:
< 1:10 No antibody detected.
Interpretive Data: A titer of greater than or equal to 1:10 indicates antibody was detected to either ANNA-1 (anti-Hu) or ANNA-2 (anti-Ri). An immunoblot assay should be completed for confirmation of ANNA-1 or ANNA-2.

Refer to Statement D under Testing Information at http://www.aruplab.com.
Note: Titers of greater than or equal to 1:10 indicate antibody was detected to either ANNA-1 (anti-Hu) or ANNA-2 (anti-Ri) and will be titered to endpoint.  This test is used primarily for titering patient sera that have previously been confirmed as positive for either ANNA-1 or ANNA-2 by immunoblot.  Only an immunoblot can differentiate between ANNA-1 and ANNA-2.
CPT Code(s): 86256