ARUP's Laboratory Test Directory

Neuronal Nuclear Antibody (ANNA) IgG by IFA with Reflex to Titer & Immunoblot, Serum : 0050904

Mnemonic: ANNAR

Methodology: Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Immunoblot
Performed: Wed
Reported: 1-9 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature: Frozen.

Unacceptable Conditions: Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 1 year
Reference Interval:
Test Number Components Reference Interval
Neuronal Nuclear Antibody (ANNA) IgG Screen, by IFA < 1:10
0050892Neuronal Nuclear Antibody (ANNA) Reflex IFA Titer, IgG < 1:10 No antibody detected
0051088Neuronal Nuclear Antibodies (ANNA) IgG, Immunoblot, Serum
Test Number Components Reference Interval
Neuronal Nuclear Antibody IgG, Immunoblot Anti-Hu Refer to report
Neuronal Nuclear Antibody IgG, Immunoblot Anti-Ri Refer to report

Interpretive Data: ANNA-1 (anti-Hu) and ANNA-2 (anti-Ri) are screened by immunofluorescence and confirmed by immunoblot detection of antibody reactive with a 35-40 kDa (ANNA-1) or a 55 kDa (ANNA-2) neuronal protein. ANNA-1 is present in patients with paraneoplastic syndromes (paraneoplastic sensory neuropathy or paraneoplastic encephalomyelitis) most commonly associated with small-cell lung carcinoma (SCLC). ANNA-2 is present in patients with midbrain encephalitis, cerebellar ataxia, eye movement disorders (usually opsoclonus), and occasionally myelopathy. Neoplasms most often associated with ANNA-2 are breast carcinoma and SCLC. ANNA-1 and ANNA-2 may be detectable before the associated neoplasm is detectable.

ANNA antibodies are screened by IFA. If the IFA screen shows reactivity at 1:10, the specimen will be titered to endpoint and confirmed (Hu or Ri) by immunoblot.

Refer to Statement D under Testing Information at http://www.aruplab.com.
Note: ANNA antibodies are screened by IFA. If the IFA screen is 1:10, then a titer and immunoblot (Hu and Ri) will be added. Additional charges apply.
CPT Code(s): 86255 ANNA; if reflexed, add 86256 ANNA titer and 83516 Immunoblot
 
 

 

 

 
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