ARUP's Laboratory Test Directory

Extractable Nuclear Antigen Antibodies (SSA & SSB) : 0050791

Mnemonic: SSA/SSB

Methodology: Semi-Quantitative Multiplex Bead Assay
Performed: Sun-Sat
Reported: Refer to individual components
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
 
Test Number Components Reference Interval
0050691SSA (Ro) (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
0050692SSB (La) (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Interpretive Data: SSA (Ro) antibody is seen in 70-75% of Sjögren syndrome cases, 30-40% of systemic lupus erythematosus (SLE) and 5-10% of progressive systemic sclerosis (PSS).  SSB (La) antibody is seen in 50-60% of Sjögren syndrome cases and is specific if it is the only ENA antibody present. Fifteen to 25% of patients with systemic lupus erythematosus (SLE) and 5-10% of patients with progressive systemic sclerosis (PSS) also have this antibody.
CPT Code(s): 86235 x2
Cross References: Sjögren Syndrome (Extractable Nuclear Antigen Antibodies (SSA & SSB))