ARUP's Laboratory Test Directory

Protein Electrophoresis with Reflex to Immunofixation Electrophoresis Monoclonal Protein Detection, Quantitation & Characterization, IgA, IgG, & IgM, Serum : 0050658

Mnemonic: SPE REFLEX

Methodology: Capillary Electrophoresis/Immunofixation Electrophoresis/Nephelometry
Performed: Mon-Fri
Reported: 1-5 days
Specimen Required: Collect:  One 4 mL serum separator tube.

Transport:  1 mL serum at 2-8°C. (Min: 0.5 mL)  Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport:  0.5 mL serum at 2-8°C.

Remarks:  Separate serum from cells ASAP.

Unacceptable Conditions:  Plasma.

Stability:  After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month
Reference Interval:

 


                                                       
 
Test Number Components Reference Interval
0050640Protein Electrophoresis, Serum  
 
Test Number Components Reference Interval
 Total Protein-Electrophoresis, Serum Refer to test 0050545
 Albumin Refer to test 0050020
 Alpha-1 Globulins Refer to test 0050035
 Alpha-2 Globulins Refer to test 0050060
 Beta Globulins Refer to test 0050110
 Gamma  Refer to test 0050270
 Immunoglobulin G, Serum 0 to 1 month: 611-1542 mg/dL      9-11 months: 282-1026 mg/dL
1 month: 241-870 mg/dL                    1 year: 331-1164 mg/dL
2 months: 198-577 mg/dL                  2 years: 407-1009 mg/dL
3 months: 169-558 mg/dL                  3 years: 423-1090 mg/dL
4 months: 188-536 mg/dL                  4 years: 444-1187 mg/dL
5 months: 165-781 mg/dL                  5-7 years: 608-1229 mg/dL
6 months: 206-676 mg/dL                  8-9 years: 584-1509 mg/dL
7-8 months: 208-868 mg/dL              10 years & older: 768-1632 mg/dL
 Immunoglobulin A, Serum 0-30 days: 1-7 mg/dL                       
1 month: 1-53 mg/dL                                   
2 months: 3-47 mg/dL                                 
3 months: 5-46 mg/dL                             
4 months: 4-72 mg/dL                             
5 months: 8-83 mg/dL                                 
6 months: 8-67 mg/dL                             
7-8 months: 11-89 mg/dL 
9-11 months: 16-83 mg/dL
1 year: 14-105 mg/dL
2 years: 14-122 mg/dL
3 years: 22-157 mg/dL
4 years: 25-152 mg/dL
5-7 years: 33-200 mg/dL
8-9 years: 45-234 mg/dL
10 years and older: 68-378 mg/dL   
 Immunoglobulin M, Serum 0-30 days: 0-24 mg/dL                     
1 month: 19-83 mg/dL                               
2 months: 16-100 mg/dL                         
3 months: 23-85 mg/dL                               
4 months: 26-96 mg/dL                             
5 months: 31-103 mg/dL                           
6 months: 33-97 mg/dL                               
7-8 months: 32-120 mg/dL 
9-11 months: 39-142 mg/dL
1 year: 41-164 mg/dL
2 years: 46-160 mg/dL
3 years: 45-190 mg/dL
4 years: 41-186 mg/dL
5-7 years: 46-197 mg/dL
8-9 years: 49-230 mg/dL
10 years and older: 60-263 mg/dL
 
Interpretive Data: Caution: Immunofixation electrophoresis is a more sensitive technique than serum protein electrophoresis for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma and macroglobulinemia, solitary plasmacytoma, and extramedullary plasmacytoma.
Note: Serum protein patterns with monoclonal or suspicious patterns will be tested by immunofixation electrophoresis which is more sensitive in determining and defining monoclonal proteins.  Patterns which are normal, polyclonal, or lacking monoclonal patterns will not be tested by IFE.
CPT Code(s): 84160 Total protein; 84165 Electrophoretic fractionation; if reflexed add 82784 x3 IgA, IgG, and IgM and 86334 IFE
 
 

 

 

 
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