ARUP's Laboratory Test Directory

0050618: Bence Jones Protein, Quantitative Free Kappa & Lambda Light Chains, Urine

Test Mnemonic: BJ QUANT
Methodology: Immunofixation Electrophoresis/Nephelometry

Performed: Mon-Fri

Reported: 1-5 days

Specimen Required:  
Collect: 24-hour urine. Urine supernate is also acceptable.  Specimen needs to be refrigerated during collection.

Transport: Two 4.5 mL aliquots from a well-mixed 24-hour collection at 2-8°C. (Min: 4.5 mL) Submit specimen in two ARUP Standard Transport Tubes.

Pediatric Collection/Transport: 2.5 mL aliquot from a well-mixed 24-hour urine collection at 2-8°C.

Remarks: Keep refrigerated at all times. Record total volume and collection time interval on transport tube and test request form.  Random urine specimens are acceptable, but not preferred.

Unacceptable Conditions: Specimens that are not refrigerated.

Stability: Ambient: 2 hours; Refrigerated: 1 week; Frozen: 1 month

Reference Interval:

 
 
Test Number Components Reference Interval
 Total Protein 10-140 mg/d
 Albumin, Urine Detected
 Alpha-1 Globulins, Urine None detected
 Alpha-2 Globulins, Urine None detected
 Beta Globulins, Urine None detected
 Gamma, Urine None detected
 Free Urinary Kappa Light Chains 0.14 - 2.42 mg/dL
 Free Urinary Kappa Excretion/Day By report
 Free Urinary Lambda Light Chain 0.02 - 0.67 mg/dL
 Free Urinary Lambda Excretion/Day By report
 Free Urinary Kappa/Lambda Ratio 2.04 - 10.37 (ratio)
 IFE Interpretation By report

Interpretive Data:
Total urinary protein is determined nephelometrically by adding the albumin and kappa and/or lambda light chains. This value may not agree with the total protein as determined by chemical methods, which characteristically underestimate urinary light chains.



CPT Code(s):
84156 Total protein; 86335 IFE; 83883 x2 Nephelometry, kappa/lambda

 

 

 
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