ARUP's Laboratory Test Directory

Kappa and Lambda Free Light Chains (Bence Jones Protein), Quantitative, Urine : 0050618

Mnemonic: BJ QUANT

Ordering Recommendation: Aids in the diagnosis and management of plasma cell dyscrasia. Preferred test to detect and quantify urinary free light chains (Bence Jones protein).
Methodology: Qualitative Immunofixation Electrophoresis/Quantitative Nephelometry
Performed: Mon-Fri
Reported: 1-5 days
Specimen Required: Collect: 24-hour urine. Refrigerate during collection. Also acceptable: Random urine specimens and urine supernate.

Specimen Preparation: Transfer two 4 mL aliquots from a well-mixed 24-hour collection to individual ARUP Standard Transport Tubes. (Min: 3 mL) Record total volume and collection time interval on transport tube and test request form.

Storage/Transport Temperature: Refrigerated.

Stability (collection to initiation of testing): Ambient: 2 hours; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval:
 
Test Number Components Reference Interval
 Total Protein 10-140 mg/d
 Albumin, Urine Detected
 Alpha-1 Globulins, Urine None detected
 Alpha-2 Globulins, Urine None detected
 Beta Globulins, Urine None detected
 Gamma, Urine None detected
 Free Urinary Kappa Light Chains 0.14 - 2.42 mg/dL
 Free Urinary Kappa Excretion/Day By report
 Free Urinary Lambda Light Chain 0.02 - 0.67 mg/dL
 Free Urinary Lambda Excretion/Day By report
 Free Urinary Kappa/Lambda Ratio 2.04 - 10.37 (ratio)
 IFE Interpretation By report

Interpretive Data: Total urinary protein is determined nephelometrically by adding the albumin and kappa and/or lambda light chains. This value may not agree with the total protein as determined by chemical methods, which characteristically underestimate urinary light chains.
CPT Code(s): 84156; 86335; 83883 x2
Cross References: Electrophoresis, Protein (Bence Jones Protein, Quantitative Free Kappa & Lambda Light Chains, Urine), Free Kappa & Lambda Light Chains, IFE (Bence Jones Protein, Quantitative Free Kappa & Lambda Light Chains, Urine) , Monoclonal Protein Study, Random Urine (Bence Jones Protein, Quantitative Free Kappa & Lambda Light Chains, Urine) , Protein Electrophoresis (Bence Jones Protein, Quantitative Free Kappa & Lambda Light Chains, Urine)