ARUP's Laboratory Test Directory

Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility : 0050610

Mnemonic: HGBEL

Methodology: High Performance Liquid Chromatography/Electrophoresis/RBC Solubility
Performed: Sun-Sat
Reported: 1-7 days
Specimen Required: Collect:  One 5 mL lavender (EDTA) or pink (K2EDTA). 

Transport:  5 mL whole blood at 2-8°C. (Min: 1 mL)

Pediatric Collection/Transport:  0.2 mL whole blood (lavender, EDTA) at 2-8°C.

Unacceptable Conditions:  Ambient and frozen samples.

Stability:  Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval:
 
Age-Defined Normal Hemoglobin Reference Intervals
Age Hb A % Hb A2 % Hb F % Hb S % Hb C % Hb E % Hb Other %
0-1 month
2 months
3 months
4 months
5 months
6-8 months
9-12 months
13-23 months
2 years and older 		17.7-54.0
37.1-70.6
41.0-84.0
68.2-88.6
74.9-95.6
83.5-95.8
91.7-96.7
94.5-98.2
94.3-98.5 		0.0-1.3
0.4-1.9
1.0-3.0
2.0-2.8
2.1-3.1
1.9-3.5
2.0-3.3
1.6-3.5
1.5-3.7 		46.0-81.0
29.0-61.0
15.0-56.0
9.4-29.0
2.3-22.0
2.3-13.0
1.3-5.0
0.2-2.0
0.0-2.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

Note: If HPLC detects any abnormal peaks suggestive of a hemoglobin variant, then further testing (RBC Solubility, Acid or Alkaline Electrophoresis) will be performed to aid in identification and confirmation.

Recommend quantitation of hemoglobin for definitive diagnosis after 1 year of age.  Abnormal hemoglobin variants may require additional testing, up to 10 days.
CPT Code(s): 83021 Chromatography; 83020 Electrophoresis and/or 85660 Sickling of RBC, reduction
 
 

 

 

 
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