ARUP's Laboratory Test Directory

Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility : 0050610
[ image for: Patient History for Hemoglobinopathy/Thalassemia Testing]
Patient History for Hemoglobinopathy/Thalassemia Testing
  


Mnemonic: HGBEL

Methodology: High Performance Liquid Chromatography/Electrophoresis/RBC Solubility
Performed: Sun-Sat
Reported: 1-7 days
Specimen Required: Collect: Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation: Transport 5 mL whole blood. (Min: 0.2 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Frozen or room temperature specimens.

Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval:
 
Age-Defined Normal Hemoglobin Reference Intervals
Age Hb A % Hb A2 % Hb F % Hb S % Hb C % Hb E % Hb Other %
0-1 month
2 months
3 months
4 months
5 months
6-8 months
9-12 months
13-23 months
2 years and older 		17.7-54.0
37.1-70.6
41.0-84.0
68.2-88.6
74.9-95.6
83.5-95.8
91.7-96.7
94.5-98.2
94.3-98.5 		0.0-1.3
0.4-1.9
1.0-3.0
2.0-2.8
2.1-3.1
1.9-3.5
2.0-3.3
1.6-3.5
1.5-3.7 		46.0-81.0
29.0-61.0
15.0-56.0
9.4-29.0
2.3-22.0
2.3-13.0
1.3-5.0
0.2-2.0
0.0-2.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0 		0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

Note: If HPLC detects any abnormal peaks suggestive of a hemoglobin variant, then further testing (RBC Solubility, Acid or Alkaline Electrophoresis) will be added to aid in identification and confirmation. Additional charges apply. 

In infants age 1 year and older, quantitation of hemoglobin is recommended for definitive diagnosis. Abnormal hemoglobin variants may require additional testing, up to 10 days.
CPT Code(s): 83021 Chromatography; 83020 Electrophoresis and/or 85660 Sickling of RBC, reduction
Cross References: Evaluation, Hemoglobin (Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility), Fetal Hemoglobin (Hemoglobin F) (Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility) , Sickle Cell Anemia Screen (Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility) , Sickle Cell Disease (Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility), Sickle Cell Screen (Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility)
 
 

 

 

 
All ARUP Sites:        www.aruplab.com     ·     www.arupconsult.com     ·     www.arup.utah.edu     ·     www.childx.org     ·     www.utahblood.org