ARUP's Laboratory Test Directory

Coccidioides Antibodies Panel, Serum by CF, ID, ELISA : 0050588

Mnemonic: COCCI PAN

Methodology: Semi-Quantitative Complement Fixation/Qualitative Immunodiffusion/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed: Refer to individual components
Reported: 2-5 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer two 1 mL aliquots of serum to individual ARUP Standard Transport Tube. (Min: 0.3 mL per aliquot) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
 
Test Number Components Reference Interval
0050170Coccidioides Antibody by CF < 1:2
0050183Coccidioides Antibody by ID None detected.
0050179Coccidioides Antibody, IgG by ELISA 0.9 IV or less: Negative- No significant level of Coccidioides IgG antibody detected.
1.0-1.4 IV: Equivocal - Questionable presence of Coccidioides IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
1.5 IV or greater: Positive - Presence of IgG antibody to Coccidioides detected, suggestive of current or past infection.
0050178Coccidioides Antibody, IgM by ELISA 0.9 IV or less: Negative - No significant level of Coccidioides IgM antibody detected.
1.0-1.4 IV: Equivocal - Questionable presence of Coccidioides IgM antibody detected.  Repeat testing in 10-14 days may be helpful.
1.5 IV or greater: Positive - Presence of IgM antibody to Coccidioides detected, suggestive of current or recent infection.

Interpretive Data: Refer to individual components.
Note: For Coccidioides, immunodiffusion (ID) measures IgM antibodies, while complement fixation (CF) measures both IgG and IgM. ELISA tests can be used to detect both coccidioidal IgG and IgM antibodies. While elevated single antibody titers may be diagnostic, paired specimens are preferred.  Acute and convalescent specimens (drawn at least 21 days apart), showing a fourfold or greater rise in titer, are diagnostic.

Negative fungal serology does not rule out the possibility of current infection.
CPT Code(s): 86635 x4
Cross References: San Joaquin Fever Antibodies (Coccidioides Antibodies Panel, Serum by CF, ID, ELISA) , Valley Fever Antibodies (Coccidioides Antibodies Panel, Serum by CF, ID, ELISA)