ARUP's Laboratory Test Directory

0050542: Haemophilus influenzae b Antibody, IgG

Test Mnemonic: HIBE IGG
Methodology: Multi-Analyte Fluorescent Detection

Performed: Mon-Fri

Reported: 1-4 days

Specimen Required:  
Collect: One 4 mL SST.

Transport: 1 mL serum at 2-8°C. (Min: 0.5 mL)  Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport: 0.15 mL serum at 2-8°C.

Remarks: Separate serum from cells ASAP. "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing. "Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen. PLEASE CLEARLY MARK SPECIMENS "PRE-VACCINE" OR "POST-VACCINE" SO THAT SPECIMENS WILL BE SAVED AND TESTED SIMULTANEOUSLY.

Unacceptable Conditions: Plasma or other body fluids.  Severely lipemic, contaminated, or hemolyzed specimens.

Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval:
< 1.0 µg/mL = Antibody concentration not protective.
³ 1.0 µg/mL = Antibody to H. influenzae b detected. Suggestive of protection.

Interpretive Data:
Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows:
1.  If the post-vaccination concentration is less than 3.0 µg/mL, the patient is considered to be a nonresponder.
2.  If the post-vaccination concentration is greater than or equal to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.



CPT Code(s):
86317