ARUP's Laboratory Test Directory

Coxiella burnetii (Q-Fever) Antibody IgG, Phase I & II by IFA : 0050462

Mnemonic: Q-F 1,2

Methodology: Semi-Quantitative Indirect Fluorescent Antibody
Performed: Tue, Fri
Reported: 1-5 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" and "convalescent."

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Contaminated, hemolyzed, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
Phase I: < 1:16 No antibody detected.
Phase II: < 1:16 No antibody detected.
Interpretive Data: Single phase II IgG titers of 1:256 and greater are considered evidence of C. burnetii infection at some time prior to the date of the serum specimen. Phase I antibody titers of 1:16 and greater are consistent with chronic infection or convalescent phase of Q-fever.
CPT Code(s): 86638 x2
Cross References: Q-Fever Antibody (Coxiella burnetii (Q-Fever) Antibody IgG, Phase I & II by IFA)