ARUP's Laboratory Test Directory
| 0050444: AML1-ETO, t(8;21) Translocation by RT-PCR |
| Test Mnemonic: AML1-ETO | |
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Methodology: Reverse Transcription Polymerase Chain Reaction
Performed: RNA isolation: Sun-Sat; Assay: Varies Reported: 5-10 days |
| Specimen Required: | |
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Collect: One 5 mL lavender (EDTA) or pink (K2EDTA). Also acceptable: yellow (ACD Solution A), lt. blue (sodium citrate), or green (sodium or lithium heparin).
Transport: 5 mL whole blood or bone marrow at 2-8°C. (Min: 1 mL) Remarks: Specimens must be received within 48 hours of collection due to lability of RNA. Unacceptable Conditions: Frozen specimens. Stability: Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable |
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| Reference Interval: |
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Negative: t(8;21) fusion transcript is not detected. There is no evidence of the t(8;21) by RT-PCR analysis. A negative result does not entirely exclude the presence of the t(8;21). Positive: t(8;21) fusion transcript is detected. There is evidence of the t(8;21) by RT-PCR analysis. |
| Interpretive Data: | |
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Patient RNA is isolated, reverse transcribed, and subjected to PCR amplification using specific oligonucleotide primers. An additional PCR amplification directed at a housekeeping gene transcript is performed as a control for the sample cDNA quality. Each assay includes a positive control reaction using cDNA from a sample or cell line known to contain the t(8;21) and a negative control reaction using cDNA from a reactive tissue sample or cell line with no evidence of t(8;21). PCR products are detected by fluorescence using a specific pair of hybridization probes. The limit of detection for this assay is 1 in 500 cells. Results of this test must always be interpreted in the context of morphologic and other relevant data, and should not be used alone for a diagnosis of malignancy. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc. Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| CPT Code(s): | |
| 83891 Isolation; 83902 Reverse transcription; 83898 x2 Amplification; 83896 x4 Nucleic acid probes; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders. |