ARUP's Laboratory Test Directory

RhEe Antigen (RHCE) Genotyping : 0050423
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Time Sensitive
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Patient History for Fetal Molecular Testing


Mnemonic: RH E

Methodology: Polymerase Chain Reaction/Fluorescent Monitoring
Performed: Tue, Thu, Sat
Reported: 2-7 days
Specimen Required: Collect:  Cultured cells (preferred): Two T-25 flasks at 80% confluent of cultured amniocytes. Fill flasks with culture media to ship at 20-25°C.  Backup cultures must be retained at the client's institution until testing is complete. If the client is unable to culture amniocytes, this can be arranged by contacting ARUP Client Services at (800) 522-2787.  Amniotic fluid: 10 mL unspun fluid at 20-25°C. (Min: 5 mL)

Remarks:  Fetal samples are CRITICAL AMBIENT and must be received within 48 hours of shipment due to lability of cells. 

Stability:  Ambient: 2 days; Refrigerated: 2 days; Frozen: Unacceptable
Reference Interval:
Homozygous Rh EE:  This sample has two copies of Rh E allele.
Heterozygous Rh Ee: This sample has one copy of Rh E and one copy of Rh e.
Homozygous Rh ee:  This sample has two copies of Rh e allele.
Interpretive Data: This DNA test (PCR) determines the rhesus type at the Rh E/e locus for a fetus at risk for erythroblastosis due to isoimmunization. Maternal-fetal Rh E/e antigen incompatibility can cause alloimmune hemolytic disease in the fetus or newborn. Fetuses predicted not at-risk for maternal-fetal incompatibility at the Rh E/e locus should continue to be monitored using clinical and non-invasive means (such as sonography and monitoring of maternal antibody titer) for the development of erythroblastosis and hydrops. The actual error rate of typing amniotic fluid for Rh E/e is unknown. Bloody amniotic fluid samples may give false-negative results due to maternal cell contamination. Please interpret results in conjunction with clinical findings. 

Samples are analyzed for the Rh E/e polymorphisms (exon 5) in the Rh CE gene by PCR and fluorescent monitoring using hybridization probes. The reliability of this test is believed to exceed 99%.  Specificity may be compromised by PCR primer site mutations or other mutations within the probe region.

For quality assurance purposes, ARUP Laboratories will provide a confirmation of the above result at no charge.  Following delivery, please collect a cord blood sample from the infant in a lavender (EDTA) or yellow (ACD Solution A) tube.  Please specify on the test request form that this is a confirmatory study to be performed at no charge. Please provide the mother's name for specimen identification purposes.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.





Please refer to Statement C in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: For the Delta OD 450 nm, refer to Amniotic Bilirubin Scan (0080276).
CPT Code(s): 83890 Extraction; 83896 x2 Nucleic acid probes; 83898 Amplification; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.
 
 

 

 

 
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