#ExistMethodology>
| Methodology: |
Polymerase Chain Reaction/Fluorescent Monitoring
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*ExistMethodology>
#ExistPerformed>
| Performed: |
Tue, Thu, Sat
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*ExistPerformed>
#ExistReported>
| Reported: |
2-7 days
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*ExistReported>
#ExistSpecRequirements>
| Specimen Required: |
#ExistCollect>Collect: Fetal Specimen: Two T-25 flasks at 80% confluency of cultured amniocytes. If the client is unable to culture amniocytes, this can be arranged by contacting ARUP Client Services at (800) 522-2787. Or amniotic fluid. AND Maternal Cell Contamination Specimen: Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).
OR Whole Blood in lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).
*ExistCollect>
#ExistSPECIMENPREPARATION>Specimen Preparation: Cultured Amniocytes: Fill flasks with culture media. Transport two T-25 flasks at 80% confluency of cultured amniocytes filled with culture media. Backup cultures must be retained the client's institution until testing is complete. OR Amniotic Fluid: Transport 10 mL unspun fluid. (Min: 5 mL) AND Maternal Cell Contamination Specimen: Transport 3 mL whole blood (Min: 1 mL)
OR Whole Blood: Transport 3 mL whole blood (Min: 1 mL)
*ExistSPECIMENPREPARATION>
#ExistSPECTRANSPORT>Storage/Transport Temperature: Cultured Amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to liability of cells. Amniotic fluid: Room temperature. Whole Blood or Maternal Cell Contamination Specimen: Room temperature.
*ExistSPECTRANSPORT>
#ExistRemarks>Remarks: Maternal specimen is recommended for proper test interpretation. Order Maternal Cell Contamination. Patient History Form is available on the ARUP Web site or by contacting ARUP Client Services.
*ExistRemarks>
#ExistStability>Stability (collection to initiation of testing): Fetal Specimen: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable Whole Blood or Maternal Cell contamination Specimen: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable *ExistStability>
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*ExistSpecRequirements>
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