ARUP's Laboratory Test Directory

Legionella pneumophila Antibody (Types 1-6), IgG by IFA : 0050365

Mnemonic: LEGAB

Methodology: Indirect Fluorescent Antibody
Performed: Mon-Fri
Reported: 1-4 days
Specimen Required: Collect:  One 4 mL serum separator tube.

Transport:  1 mL serum at 2-8°C. (Min: 0.5 mL)  Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport:  0.15 mL serum at 2-8°C.

Remarks:  Separate serum from cells ASAP. Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Please mark specimen plainly as "acute" or "convalescent."

Unacceptable Conditions:  Severely lipemic, contaminated, or hemolyzed specimens.

Stability:  After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
<1:128  Negative -  No significant level of Legionella pneumophila Type 1-6 IgG antibody detected.
1:128 Equivocal - Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1:256 or greater Positive -  Presence of Legionella pneumophila Type 1-6 IgG antibody detected, suggestive of current or past infection.
Interpretive Data: Seroconversion between acute and convalescent sera is considered strong evidence of recent infection.  The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.  This assay may detect infection by any of the serotypes 1-6.  The CDC and many state health laboratories recommend testing only for antibody to Legionella pneumophila Type 1.  For equivocal or positive IFA results, the CDC protocol suggests follow-up testing for Legionella pneumophila antibody Type 1.
Note: For follow-up testing on equivocal or positive results, refer to Legionella pneumophila Antibody (Type 1), IgG by IFA (0050376).
CPT Code(s): 86713
 
 

 

 

 
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