ARUP's Laboratory Test Directory

Borrelia burgdorferi Antibodies, IgG & IgM by Western Blot : 0050254

Mnemonic: LYME WB

Methodology: Western Blot
Performed: Sun, Tue, Thu, Fri
Reported: 1-3 days
Specimen Required: Collect:  One 4 mL serum separator tube.

Transport:  1 mL serum at 2-8°C. (Min: 0.2 mL)  Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport:  0.1 mL serum at 2-8°C.

Remarks:  Separate serum from cells ASAP. 

Unacceptable Conditions:  CSF or plasma.  Severely lipemic, heat-inactivated, contaminated, or hemolyzed specimens.

Stability:  After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
Negative

IgG Positive: Any five of the following 10 bands: 18, 23, 28, 30, 39, 41, 45, 58, 66, 93 kDa.
IgG Negative: Any pattern that does not meet the IgG-positive criteria.

IgM Positive: Any two of the following three bands: 23, 39, 41 kDa.
IgM Negative: Any pattern that does not meet the IgM-positive criteria.
Note: Per CDC guidelines, if ELISA test result is NEGATIVE, Western blot should not be performed.

This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi total antibodies, IgG and/or IgM test performed on patients less than four weeks after appearance of erythema migrans.
CPT Code(s): 86617 Lyme IgG; 86617 Lyme IgM
 
 

 

 

 
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