ARUP's Laboratory Test Directory
| 0050254: Borrelia burgdorferi Antibodies, IgG & IgM by Western Blot |
| Test Mnemonic: LYME WB | |
|
Methodology: Western Blot
Performed: Sun, Tue, Thu, Fri Reported: 1-3 days |
| Specimen Required: | |
|
Collect: One 4 mL SST.
Transport: 1 mL serum at 2-8°C. (Min: 0.2 mL) Submit specimen in an ARUP Standard Transport Tube. Pediatric Collection/Transport: 0.1 mL serum at 2-8°C. Remarks: Separate serum from cells ASAP. Unacceptable Conditions: CSF or plasma. Severely lipemic, heat-inactivated, contaminated, or hemolyzed specimens. Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) |
|
| Reference Interval: |
|
Negative IgG Positive: Any five of the following 10 bands: 18, 23, 28, 30, 39, 41, 45, 58, 66, 93 kDa. IgG Negative: Any pattern that does not meet the IgG-positive criteria. IgM Positive: Any two of the following three bands: 23, 39, 41 kDa. IgM Negative: Any pattern that does not meet the IgM-positive criteria. |
| Note: | |
|
Per CDC guidelines, if ELISA test result is NEGATIVE, Western blot should not be performed. This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi total antibodies, IgG and/or IgM test performed on patients less than four weeks after appearance of erythema migrans. |
| CPT Code(s): | |
| 86617 Lyme IgG; 86617 Lyme IgM |