ARUP's Laboratory Test Directory

0050249: Enterovirus Detection by RT-PCR

Test Mnemonic: EV PCR
Methodology: Reverse Transcription/Polymerase Chain Reaction

Performed: Sun-Sat

Reported: 1-2 days

Specimen Required:  
Collect: One 5 mL lavender (EDTA) or pink (K2EDTA), or CSF. Spin blood sample and remove plasma before transporting.  Specimen source is required.

Transport: 1 mL plasma or CSF in a sterile container, frozen.

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Pediatric Collection/Transport: 0.25 mL plasma or CSF in a sterile container, frozen.  (Less volume may decrease sensitivity of assay.)

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Remarks: Avoid repeated freeze/thaw cycles.

Unacceptable Conditions: Nonfrozen samples, samples exposed to repeated freeze/thaw cycles, nonsterile or leaking containers, heparinized samples, and hemolyzed samples.

Stability: Ambient: 1 hour; Refrigerated: 24 hours; Frozen: 3 months

Reference Interval:
Negative - Enterovirus nucleic acid not detected by RT-PCR.
Positive - Enterovirus nucleic acid detected by RT-PCR.

Interpretive Data:
A negative result does not rule out the presence of PCR inhibitors in the patient specimen or Enterovirus nucleic acid in concentrations below the level of detection of the assay.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory.



CPT Code(s):
87498