ARUP's Laboratory Test Directory

West Nile Virus RNA by RT-PCR : 0050229

Mnemonic: WNILE PCR

Methodology: Reverse Transcription Polymerase Chain Reaction
Performed: Mon-Fri
Reported: 1-4 days
Specimen Required: Collect:  One 5 mL plain red, serum separator tube or CSF.  (Centrifuge blood sample and remove serum before transporting.)  Specimen source is required.

Transport:  1 mL serum or CSF in a sterile container, frozen.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Pediatric Collection/Transport:  0.3 mL serum or CSF in a sterile container, frozen.  Less volume may decrease sensitivity of assay.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks:  Avoid repeat freeze/thaw cycles.

Unacceptable Conditions:  Heparinized or hemolyzed samples, and nonsterile or leaking containers.

Stability:  Ambient: 1 hour; Refrigerated: 24 hours; Frozen: 3 months
Reference Interval:
Negative: West Nile virus nucleic acid not detected by RT-PCR.
Positive: West Nile virus nucleic acid detected by RT-PCR.
Interpretive Data: A negative result does not rule out the presence of PCR inhibitors in the patient specimen or West Nile virus nucleic acid in concentrations below the level of detection of the assay.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
CPT Code(s): 87798
 
 

 

 

 
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