ARUP's Laboratory Test Directory
| 0050215: dsDNA (Double Stranded DNA) Antibody, IgG |
| Test Mnemonic: DNA | |
|
Methodology: Enzyme-Linked Immunosorbent Assay/Indirect Fluorescent Antibody
Performed: Sun-Sat Reported: 1-3 days |
| Specimen Required: | |
|
Collect: One 4 mL SST.
Transport: 1 mL serum at 2-8°C. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube. Pediatric Collection/Transport: 0.15 mL serum at 2-8°C. Remarks: Separate serum from cells ASAP. Unacceptable Conditions: Plasma. Severely lipemic, contaminated, or hemolyzed specimen s. Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen 1 year (avoid repeated freeze/thaw cycles) |
|
| Reference Interval: |
| None detected. |
| Interpretive Data: | |
|
dsDNA antibodies are screened using an ELISA assay. Positive results are titered by IFA. "None detected" correlates with an IFA titer of less than 1:10.
|
| Note: | |
| dsDNA antibodies (1:10 or greater) are found in 50-60% of systemic lupus erythematosus (SLE), 20-30% in Sjögren syndrome, 20-25% in mixed connective tissue disease (MCTD), and less than 5% in progressive systemic sclerosis (PSS). High titers of antibody to native (double stranded) DNA are specific for SLE. |
| CPT Code(s): | |
| 86225 |