Ordering Recommendation

Confirm a prior infection with group A Streptococcus in patients suspected of having a nonsuppurative complication such as acute glomerulonephritis (AGN) or acute rheumatic fever (ARF). DNase-B Antibody (0050220) and Streptolysin O Antibody (ASO) (0050095) antibody tests are generally ordered concurrently.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP standard transport tube. (Min: 0.4 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed specimens.

Remarks
Stability

After separation from cells: Ambient: 8 hours; Refrigerated: 8 days; Frozen: 3 months

Methodology

Quantitative Nephelometry

Performed

Sun-Sat

Reported

Within 24 hours

Reference Interval

0-1 year: Less than 200 IU/mL
2-12 years: Less than 240 IU/mL
13 years and older: Less than 330 IU/mL

Interpretive Data

Elevated titers of antideoxyribonuclease B antibody (anti-DNase B) or antistreptolysin O antibody (ASO) indicate a recent group A Streptococcus infection. Anti-DNase B antibodies typically remain elevated longer than ASO and may remain elevated for several months after infection. Patients suspected of having complications related to a recent Streptococcus infection such as acute glomerulonephritis or acute rheumatic fever may have elevated anti-DNase B but normal ASO antibody titers. A negative or very low anti-DNase B and ASO antibody titers, especially from a specimen tested 2 weeks after a suspected infection, indicates unlikely incidence of a recent Streptococcus infection.

Compliance Category

FDA

Note

Hotline History

N/A

CPT Codes

86060

Components

Component Test Code* Component Chart Name LOINC
0050095 Streptolysin O Antibody 5370-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Anti-Streptolysin O Antibody
  • ASO antibody test
  • Streptococcal antibodies
Streptolysin O Antibody (ASO)