ARUP's Laboratory Test Directory

EGFR Gene Amplification by FISH : 0049234

Mnemonic: EGFRFISH

Methodology: Fluorescence in situ Hybridization
Performed: Weekly
Reported: 4-12 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides) Protect paraffin block from excessive heat.

Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months.

Remarks: Include surgical pathology report.

Unacceptable Conditions: Frozen specimens. Specimens fixed or processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Reference Interval:
Test Number Components Reference Interval
EGFR by FISH Nonamplified: LSI EGFR:CEP7 Less than 2.0
Amplified: LSI EGFR:CEP7 Greater than or equal to 2.0
EGFR:CEP7 By report
EGFR per Cells Counted Ratio By report

Interpretive Data: Refer to report.

Refer to Statement A under Testing Information at http://www.aruplab.com.
CPT Code(s): 88368 x2
Cross References: Her-1 (EGFR Gene Amplification by FISH)
 
 

 

 

 
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