ARUP's Laboratory Test Directory
| 0049192: Glomerular Basement Membrane Antibody, IgA (IFA) |
| Test Mnemonic: GBM-A IFA | |
|
Methodology: Indirect Fluorescent Antibody
Performed: Mon-Fri Reported: Within 5 days |
| Specimen Required: | |
|
Collect: One 4 mL SST.
Transport: 1 mL serum at 2-8°C. (Min: 0.5 mL) Pediatric Collection/Transport: 0.5 mL serum at 2-8°C. Remarks: Separate serum from cells ASAP. Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid freeze/thaw cycles) |
|
| Reference Interval: |
| Negative |
| Interpretive Data: | |
|
The anti-GBM IFA methodology has been shown to be less specific than the quantitative tests for circulating antibodies such as the Multi-Analyte Fluorescent Detection (MAFD). The antibody used in this assay, although directed to human IgG F(ab')2, can bind to light chain sites common to all immunoglobulins causing it to be more sensitive but less specific. The determination of a positive result is based upon a diffuse "linear" staining pattern as viewed through a fluorescent microscope, all positive results should be confirmed by quantitative tests and/or renal biopsy.
Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| CPT Code(s): | |
| 86255 |