| Methodology: |
Cytochemical Stain
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| Performed: |
Mon-Fri
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| Reported: |
Within 5 days
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| Specimen Required: |
Collect: Peripheral Blood: One 5 mL lavender (EDTA) or pink (K2EDTA), or one 7 mL green (sodium or lithium heparin); or Bone Marrow: 1 mL heparinized aspirate; or Paraffin: Block or four unstained paraffin slides cut at 4 microns.
Storage/Transport Temperature: Peripheral Blood: 5 mL whole blood (EDTA or green, heparin) and six unfixed smears; Bone Marrow: 1 mL heparinized aspirate and six unfixed smears. Transport peripheral blood and bone marrow at 20-25°C. Paraffin: Blocks and slides must be protected from excessive heat. Ship in cooled containers during summer months.
Minimum volume required: Peripheral Blood: 1 mL whole blood (EDTA or green heparin) and six well-prepared unfixed smears. Bone Marrow: 0.5 to 1 mL heparinized aspirate and six well-prepared unfixed smears. Paraffin: block or four unstained paraffin slides cut at 4 microns. Transport peripheral blood and bone marrow at 20-25 Degrees C. Paraffin: blocks and slides must be protected from excessive heat. Ship in cooled containers during summer months.
Remarks: Blood specimens must be received within 24 hours of collection; testing must be performed within 48 hours of collection. Protect from light.
Stability (collection to initiation of testing): Blood/Bone marrow: Ambient: 24 hours; Paraffin: Indefinitely
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