ARUP's Laboratory Test Directory

KIT (D816V) Mutation by PCR : 0040137

Mnemonic: KIT-F

Methodology: Polymerase Chain Reaction
Performed: Varies
Reported: 2-7 days
Specimen Required: Collect: Lavender (EDTA) OR bone marrow (EDTA) OR tissue.

Specimen Preparation: Whole blood: Transport 5 mL (Min: 1 mL) OR Bone marrow: Transport 3 mL (Min: 1 mL) OR Fresh tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue. OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or four 10-micron shavings. Tissue block will be returned after testing. Transport tissue (frozen, paraffin blocks) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature: Whole blood OR Bone marrow: Refrigerated. Fresh tissue: Frozen on dry ice. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions: Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.

Stability (collection to initiation of testing): Whole blood OR Bone marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Fresh Tissue: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
FFPE tumor tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 81402, G0452
Cross References: Asp816Val (KIT (D816V) Mutation by PCR, Fluid) , CKIT (KIT (D816V) Mutation by PCR, Fluid) , D816V (KIT (D816V) Mutation by PCR, Fluid) , Mast Cell Disease (KIT (D816V) Mutation by PCR, Fluid) , Systemic Mastocytosis (KIT (D816V) Mutation by PCR, Fluid)