ARUP's Laboratory Test Directory

Manual Differential : 0040005

Mnemonic: DIFF

Methodology: Microscopy
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Lavender (EDTA) or pink (K2EDTA). OR unstained whole blood smears.

Specimen Preparation: Specimens must be well mixed prior to making smears. Transport 3 mL whole blood OR 2 unstained whole blood smears. (Min: 0.5 mL OR 2 smears)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Frozen specimens. Clotted specimens.

Stability (collection to initiation of testing): Whole Blood: Ambient: 8 hours; Refrigerated: 24 hours; Frozen: Unacceptable
Smears: Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable
Reference Interval:
 
Test Number Components Reference Interval
 PMNs15 yrs & over: 45-79%
 Bands0-5%
 Lymphocytes15 yrs & over: 16-47%
 Monocytes15 yrs & over: 0-9%
 Eosinophil % 0-6%
 Basophil Percentage 0-2%
 Absolute Neutrophil Count  
 
Age Male (K/µL) Female (K/µL)
1-3 days 1.7-4.7 2.1-8.4
4-7 days 1.9-4.1 1.8-5.1
8-14 days 1.9-5.2 1.7-5.4
15-30 days 1.5-3.6 1.3-4.3
31-60 days 1.2-4.4 1.2-4.9
61-180 days 1.4-6.4 1.4-6.7
6 months-2 years 1.6-8.3 1.8-9.1
3-6 years 1.8-7.4 1.8-6.8
7-12 years 1.8-6.6 1.8-6.7
13 years and older 1.3-7.0 1.3-7.0
 Morphology Normocytic/Normochromic
 Platelet (estimate) Adequate

CPT Code(s): 85007