ARUP's Laboratory Test Directory

APC Resistance Profile with Reflex to Factor V Leiden : 0030192

Mnemonic: APC R

Ordering Recommendation: Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden mutation.
Methodology: Clotting/Polymerase Chain Reaction/Fluorescence Monitoring
Performed: Refer to individual components
Reported: 1-5 days
Specimen Required: Collect: Lt. blue (sodium citrate) AND lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation: Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)

Storage/Transport Temperature: Plasma: Frozen. Whole Blood: Refrigerated.

Unacceptable Conditions: Plasma: Non-frozen specimens.

Stability (collection to initiation of testing): Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval:
 
Test Number Components Reference Interval
0030127APC Resistance Profile Effective February 21, 2012

2.0 or greater
0097720Factor V Leiden (F5) R506Q Mutation Negative: This sample is negative for factor V Leiden, R506Q mutation.

Interpretive Data: Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.
Note: If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.
CPT Code(s): 85307;
If reflexed, add 81241
Cross References: Factor V Leiden Functional Assay with Reflex to PCR (APC Resistance Profile with Reflex to Factor V Leiden)