ARUP's Laboratory Test Directory
| 0030056: ADAMTS13 Activity |
| Test Mnemonic: ADAMTS-13 | |
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Methodology: Fluorescence Resonance Energy Transfer
Performed: Mon-Fri Reported: 1-4 days |
| Specimen Required: | |
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Collect: One 5 mL lt. blue (sodium citrate). (Min: 3 mL) Refer to the Hemostasis/Thrombosis section in the front of the Laboratory Test Directory for special collection instructions.
Transport: 1 mL platelet-poor plasma, frozen. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube. Pediatric Collection/Transport: 0.5 mL platelet-poor plasma, frozen. Remarks: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Unacceptable Conditions: Hemolyzed specimens. Serum, EDTA plasma. Stability: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 2 weeks |
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| Interpretive Data: | |
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Microtiter plates coated with monoclonal anti-ADAMTS13 antibody are used to measure ADAMTS13 activity by Fluorescence Resonance Energy Transfer (FRET). ADAMTS13 levels of less than 5% may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP). A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity. The monoclonal anti-ADAMTS13 antibody is directed against the CUB domain; therefore, certain forms of ADAMTS13 (with mutations in the CUB domains) may not be equivalently measured due to reduced binding to the antibody. Please refer to Statement D in the Compliance Statements section in the front of the Laboratory Test Directory. |
| CPT Code(s): | |
| 85246 |