ARUP's Laboratory Test Directory

ADAMTS13 Activity : 0030056

Mnemonic: ADAMTS-13

Methodology: Fluorescence Resonance Energy Transfer
Performed: Mon-Fri
Reported: 1-4 days
Specimen Required: Collect: Lt. blue (sodium citrate). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation: Transfer 1 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions: Hemolyzed specimens. Serum or EDTA plasma.

Stability (collection to initiation of testing): Ambient: 3 hours; Refrigerated: Unacceptable; Frozen: 2 weeks

Reference Interval:
Greater than or equal to 67 percent.
Interpretive Data: Microtiter plates coated with monoclonal anti-ADAMTS13 antibody are used to measure ADAMTS13 activity by Fluorescence Resonance Energy Transfer (FRET).

ADAMTS13 levels of less than 5% may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).

A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity.

The monoclonal anti-ADAMTS13 antibody is directed against the CUB domain; therefore, certain forms of ADAMTS13 (with mutations in the CUB domains) may not be equivalently measured due to reduced binding to the antibody.

See Compliance Statement D: www.aruplab.com/CS
CPT Code(s): 85397
Cross References: von Willebrand Factor Cleaving Protease (ADAMTS13 Activity)