ARUP's Laboratory Test Directory

ADAMTS13 Activity : 0030056

Mnemonic: ADAMTS-13

Methodology: Fluorescence Resonance Energy Transfer
Performed: Mon-Fri
Reported: 1-4 days
Specimen Required: Collect: One 5 mL lt. blue (sodium citrate). (Min: 3 mL) Refer to the Hemostasis/Thrombosis section in the front of the Laboratory Test Directory for special collection instructions.

Storage/Transport Temperature: 1 mL platelet-poor plasma, frozen. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube.

Remarks: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions: Hemolyzed specimens. Serum, EDTA plasma.

Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 2 weeks
Interpretive Data: Microtiter plates coated with monoclonal anti-ADAMTS13 antibody are used to measure ADAMTS13 activity by Fluorescence Resonance Energy Transfer (FRET).

ADAMTS13 levels of less than 5% may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).

A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity.

The monoclonal anti-ADAMTS13 antibody is directed against the CUB domain; therefore, certain forms of ADAMTS13 (with mutations in the CUB domains) may not be equivalently measured due to reduced binding to the antibody.

Please refer to Statement D in the Compliance Statements section in the front of the Laboratory Test Directory.
CPT Code(s): 85397
Cross References: ADAMTS13, von Willebrand factor cleaving protease
 
 

 

 

 
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