ARUP's Laboratory Test Directory

0020413: Lactate Dehydrogenase, Isoenzymes

Test Mnemonic: LDISO
Methodology: Enzymatic/Electrophoresis

Performed: Sun-Sat

Reported: 1-2 days

Specimen Required:  
Collect: One 4 mL SST.

Transport: 1 mL serum at 2-8°C. (Min: 0.6 mL) Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport: 0.5 mL serum at 2-8°C. 

Remarks: Do not freeze. Allow serum to clot completely at room temperature.  Separate serum from cells ASAP.

Unacceptable Conditions: Frozen or hemolyzed specimens.  Specimens collected with EDTA, potassium oxalate, or sodium fluoride anticoagulants.

Stability: After separation of cells: Ambient: 2 days; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval:
LD-1: 14-27%
LD-2: 29-42%
LD-3: 16-22%
LD-4: 8-15%
LD-5: 6-23%

Lactate Dehydrogenase, Total:
0 up to 30 days: 200-465 U/L
1-17 months: 200-450 U/L
18 months-10 years: 165-430 U/L
11-16 years: 127-287 U/L
17 years and older: 105-230 U/L 

Note:
Hemolyzed samples and serum which has not been separated from cells, show elevated LD-1 and LD-2.  LD-4 and LD-5 are labile at low temperatures and are erroneously low in samples that have been frozen.

CPT Code(s):
83625 LD isoenzymes; 83615 Total LD

 

 

 
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