Histochemical Stains

The ARUP Histology Laboratory performs more than 30 histochemical stains on formalin-fixed, paraffin-embedded tissues/cellblocks. These stains are used to selectively stain cells and cellular components used to characterize tissues (e.g., muscle fibers or connective tissue), to aid in identifying noncellular materials ( such as amyloid), and to help identify and classify infectious organisms.

This lab is pathologist oriented, providing a stain and return service only, without interpretation, to our client pathologists to assist in their diagnostic studies. Most stains are returned the next day. If interpretation is needed, please order a consultation.

For information concerning consultation, please call (801) 581-2507. Consultations can be sent directly to:

Surgical Pathology Department
Huntsman Cancer Hospital
1950 Circle of Hope, RM N3105
Salt Lake City, UT 84112
Phone: (801) 581-2507 or (801) 584-5078
Fax: (801) 581-7035

Please include pertinent clinical history and surgical pathology report.
Test # Test Name Test Keywords Additional Information
2005927 Special Stain, Copper (Rhodanine) Stain and return service only
2005906 Stain, Acid Fast AFB Stain and return service only.
Acid fast bacilli stain red.
2005981 Stain, Alcian Blue - Periodic Acid-Schiff (PAB) PAB Stain and return service only.
Stains acid mucosubstances blue. Neutral polysaccharides will stain magenta.
2005900 Stain, Alcian Blue pH 2.5 Stain and return service only.
Stains strongly acidic sulfated mucosubstances blue.
2005903 Stain, Alcian Blue with Hyaluronidase Stain and return service only.
Areas containing hyaluronic acid, hyaluronosulphate or chondroitin sulphates A and C are unstained. All others mucosubstances strongly stain blue.
2005909 Stain, Bielschowski Stain and return service only.
Stains axons, senile plaques, and neurofibrillary tangles black.
2005918 Stain, Calcium (Von Kossa) Von Kossa Stain and return service only.
Stains calcium salts black.
2005921 Stain, Colloidal Iron Stain and return service only.
Stains acid mucopolysaccharides and sialomucins deep blue.
2005924 Stain, Congo Red Stain and return service only.
Amyloid stains pink to red and will appear apple-green with a polarizing microscope.
2005930 Stain, Elastin Elastic van Gieson Stain Stain and return service only.
Elastic fibers will stain black and collagen will stain red.
2005936 Stain, Fite Fite Acid Fast Stain for Leprosy Stain and return service only.
Stains bacteria cell walls that are vulnerable to organic solvents. Leprosy and Nocardia will stain red.
2005939 Stain, Fontana-Masson Melanin Stain Stain and return service only.
Stains melanin and argentaffin black.
2005942 Stain, Giemsa Stain and return service only.
Stains helicobacter pylori dark blue.
2005948 Stain, Gram Modified Brown and Brenn Stain and return service only.
Stains gram positive bacteria blue. Gram negative bacteria will stain red.
2005945 Stain, Grocott’s Methenamine Silver (GMS) GMS Stain and return service only.
Stains fungi black.
2005912 Stain, Halls Bile Hall's Bilirubin Stain Stain and return service only.
Stains bile pigment green.
2005954 Stain, Iron Stain and return service only.
Stains iron pigment blue.
(Similar to Trumbell Blue stain.)
2005957 Stain, Jones Basement Membrane Stain and return service only.
Stains basement membrane black.
2005960 Stain, Luxol Fast Blue LFB Stain and return service only.
Myelinated fibers will stain blue. Neutrophils will stain pink and nerve cells will stain purple.
2005966 Stain, Melanin Bleach Stain and return service only.
This stain is usually used in conjunction with the melanin stain. If there is question that a melanin stain is positive, a melanin bleach stain will be run. The area of question on the melanin stain will be bleached out.
2005963 Stain, Modified Giemsa (Diff-Quick) Diff-Quick Stain and return service only.
Stain hematopoietic tissue.
2005972 Stain, Mucicarmine Stain and return service only.
Stains mucin deep rose.
2005978 Stain, Oil Red O Stain and return service only.
Stains fat red. This stain can only be done on frozen sections.
2005987 Stain, Periodic Acid-Schiff (PAS) with Diastase PAS-D Stain and return service only.
Glycogen will be digested out on the slides and staining will not appear as it did on a PAS slide.
2005990 Stain, Periodic Acid-Schiff (PAS), Fungus PAS-F Stain and return service only.
Stains fungus magenta.
2005993 Stain, Phosphotungstic Acid-Hematoxylin (PTAH) PTAH Stain and return service only.
Cross-striations, fibrin, and glial fibers stain blue. Collagen will stain red-brown. Elastic fibers will stain purple.
2005996 Stain, Reticulin Stain and return service only.
Stains reticular fibers black.
2005999 Stain, Steiner Stain and return service only.
Spirochetes, legionella, and campylobactor stain black.
2006002 Stain, Thioflavin S Stain and return service only.
Stains senile plaques apple green under a polarizing microscope.
2006008 Stain, Toluidine Blue Stain and return service only.
Stains mast cells violet.
2006011 Stain, Trichrome Stain and return service only.
Stains muscle red. Stains collagen blue. Stains nuclei black.
2006014 Stain, Urate Crystals Stain and return service only.
Tissue must not be processed in aqueous solutions. Urate crystals will stain black.